Ashoka Quantitative Microscopy Course

From experimental design to quantitative analysis:
A comprehensive microscopy workshop for life scientists in India

Location: Ashoka University, Sonipat, Haryana, INDIA

Course Dates: 10-16 January 2027

Application Deadline: 31 August 2026

Arrival: 10 January 2027 (Sunday)

Course: 11-15 January 2027 (Monday - Friday)

Departure: 16 January 2027 (Saturday)

Apply here

This 5-day course will equip participants to design and execute quantitative microscopy experiments to address biological questions.

Extensive hands-on microscopy practicals across a variety of
imaging platforms

This workshop will cover the following:

Microscopy
- Introduction to optics
- Fundamentals of fluorescent molecules
- Brightfield microscopy
- Widefield fluorescence microscopy
- Confocal fluorescence microscopy
- TIRF microscopy
- Light-sheet microscopy
- Two-photon microscopy
- Multispectral microscopy
- Super-resolution microscopy
  (SIM, Airyscan, STED, SMLM)
- Advanced live-cell imaging
  (FRET, FRAP, FLIP, photoactivation)

Image Processing
- Fundamentals of digital images
- Basic principles of image processing
- Fourier transformation
- Object segmentation
- Machine learning

Quantitative Analysis
- Colocalization
- Ratiometric image analysis
- Phasor analysis
- Intensity measurements
- Morphometric analysis
- Motion tracking and modeling

Participants will learn to leverage the power of advanced microscopy through informed design, execution, and reporting of quantitative, reproducible imaging experiments.

Image processing and analysis training for quantitative fluorescence microscopy

Please note:

Applicants must be from non-profit organizations in India.

All communications and interactions during the workshop will be conducted strictly in English.

Course fee is ₹5,000. Travel to Ashoka University must be self-funded by attendees. Limited travel support may be available for participants who can demonstrate financial need.