Leong became the director of the Center for Advanced Microscopy at Feinberg School of Medicine, Northwestern University in 2002, and led the facility to be recognized as one of the few selected Nikon Imaging Centers in the world. At the same time, his lab began devising methods to engineer three-dimensional, lumenized vascular network capable of dynamic signaling read-out. This approach ultimately allowed Leong and his team to dissect the regulatory signals in the opposing endothelial cells during cancer diapedesis. In 2009, he was further appointed to the position of Director for University Imaging Resources at Northwestern, overseeing the institutional strategy in building integrated imaging infrastructure across all seven imaging centers within the university.
Leong joined Janelia in 2014 to serve as the Director for the Advanced Imaging Center. Here, he leads the effort in building the unique collaborative imaging center that serves as the gateway through which the wider scientific world can access Janelia’s cutting-edge microscopy capabilities.
Jesse joined the Advanced Imaging Center in 2014, and has over 15 years’ experience in microscope design, development, and application across a wide range of biological models. He previously was Senior Engineer at Vutara, Inc., where he helped to successfully commercialize 3D super-resolution microscopy systems based on the biplane detection method. Jesse performed postdoctoral work at Sandia National Laboratories, where he implemented several high-resolution optical imaging systems, including TIRF, STORM, hyperspectral, and STED microscopies, with biological applications ranging widely from cell surface receptor tracking and innate immune response, to algal biofuels research. Jesse received his Ph.D. in Biomedical Engineering from the University of Texas at Austin.
John joined the Advanced Imaging Center in 2014 and has over a decade of experience at the interdisciplinary boundary of engineering, physics, and biology. Prior to joining the AIC, he was a National Research Council postdoctoral fellow at the National Institute of Standards and Technology (NIST) in Gaithersburg, MD. At NIST, John leveraged his expertise in cancer biology to adapt optical spectroscopy techniques for use in biological systems, including using coherent anti-stokes Raman spectroscopy (CARS) as a method of unbiased chemical analysis of normal and pathologic tissue specimens. John received his Ph.D. in Cell and Molecular Biology from Case Western Reserve University in Cleveland, Ohio where he investigated the influence of the tumor microenvironment on glioblastoma multiforme epigenetic state and tumorigenicity.
Ulrike joined the Advanced Imaging Center in 2019. She comes from a background in physics which she studied at the Technical University of Munich as well as the Max Planck Institute (MPI) of Biochemistry. At the latter she garnered advanced knowledge in correlative cryo-fluorescence/electron microscopy techniques. Thereafter, she did her PhD studies at the MPI for Biophysical Chemistry. In Göttingen, she combined two advanced imaging techniques (4Pi-RESOLFT nanoscopy) that allowed for the observation of structures within living cells at low light intensities with high isotropic spatial resolution (<40 nm) for the first time. After her PhD, Ulrike worked as a postdoctoral fellow at the National Institutes of Health. There, she studied the spatial organization of the genome with newly developed single molecule imaging strategies.
Satya has more than 30 years of cell biology experience. Combining her extensive cell biological sample prep know-how and her acumen in microscopy, Satya's work has been featured on covers of various textbooks and journals. Satya has pioneered the tissue engineering technique to create 3D, lumenized blood vessel network with FRET biosensors for the study of cancer invasion.
After serving at the Center for Advanced Microscopy at Northwestern University, she joined Janelia in 2015 as technical coordinator to provide crucial support to AIC visitors in sample receiving, cell culture, specimen preparation, and also logistical consultation on experimental set-up.
Cell Culture Technical Coordinator
Eric comes from a diverse background spanning from the arts to the sciences. He started off as a photography major and worked as a professional photographer for three years, then made a switch to the sciences where he found his passion for programming. In grad school he began applying high performance computing and 5D visualization to microscopy data. At the AIC, Eric advises visitors on which software tools are best suited to analyse their experiments. When the current suite of programs fall short, he assists visitors to create novel analysis tools and pipelines.
Mike is a recent PhD graduate from the University of Cape Town, South Africa. His PhD research involved the molecular interactions associated with the mutagenic DNA repair pathway of mycobacteria. It was during this research that Mike developed a keen interest in microscopy and imaging techniques. Before moving to the AIC, Mike assisted researchers at the University of Cape Town's Institute of Infectious Disease and Molecular Medicine conceptualize and conduct super-resolution SIM imaging experiments within a biosafety level-3 facility.
Michael is the first Advanced Imaging Fellow at the AIC and will learn the technical, theoretical, and managerial skills required to run a microscopy core facility.
Advanced Imaging Fellow
Blair joined the Advanced Imaging Center in 2019 through the Scientific Computing Associate program. Although he is officially part of Scientific Computing Software, he is fully embedded in the AIC. Blair's background is equal parts computer science and biology. He received his PhD in Computer Science from Emory University after earning a Bachelor's in Biochemistry. Prior to starting his graduate program, Blair was a Fulbright scholar at the University of Utrecht where he worked on mechanisms of asymmetric cell division in C. elegans. Blair has over ten years of experience in bioimage informatics, and he specializes in developing algorithms for spectral imaging and unmixing.
Scientific Computing Associate II
Michelle joined Janelia Research Campus in July 2012. She graduated with a B.A. in Political Science minor in Sociology from the University of the Philippines Manila after studying Nursing for two years. She currently supports the Harris, Hess, and O’Shea labs, MouseLight Project Team, as well as the Advanced Imaging Center.
Lab Administration Specialist
Meghan began her tenure at Janelia in 2013 and serves as a Manager for the Safety, Health, and Security Department. Currently, Meghan supports the AIC through conducting risk assessments and navigating United States import and shipping regulations. In addition to her support of the AIC, Meghan manages the Biological Safety, Occupational Health, and Wellness programs; serves as the executive secretary of the Janelia Institutional Biosafety Committee; and chairs the Janelia Institutional Animal Care and Use Committee. She earned her Bachelors of Science degree in Biochemistry and Molecular Biology from The Pennsylvania State University and a Doctorate of Philosophy in Cellular and Molecular Medicine from the Johns Hopkins School of Medicine. Throughout her research career, she studied transcriptional regulation, the molecular mechanisms of Parkinson’s disease, and the metabolism of brain tumors. Meghan started her safety career as a post-doctoral fellow with the National Biosafety and Biocontainment Training Program and is recognized as a Registered Biosafety Professional by ABSA International.