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Unique System Features

  • Simultaneous dual-view detection and multi-sided illumination of samples to capture embryos or other large samples in their entirety

  • 800x800 µm field of view with a Nikon 16x/0.8 NA objective or 300x300 µm field of view with a Zeiss 40x/1.0 NA objective

  • Capable of imaging up to 3 color channels (acquired sequentially) per experiment

  • Spatial resolution of 400 nm in X-Y and 2 µm in Z, isotropic spatial resolution of 400 nm possible through orthogonal multi-view imaging and multi-view deconvolution

  • Adaptive imaging framework with 10 degrees of freedom for maintaining optimal spatial resolution (by dynamically correcting for first- and second-order aberrations) and tracking specimen for the entirety of the experiment

  • Environmental chamber to ensure sample health and proper development during experiment

System Specifications

Excitation Lasers:

  • 488 nm (Omicron fiber-coupled, 200 mW maximum)

  • 561 nm (Omicron fiber-coupled, 150 mW maximum)

  • 594 nm (Omicron fiber-coupled, 100 mW maximum)

  • 642 nm (Omicron fiber-coupled, 140 mW maximum)

  • 685 nm (Omicron fiber-coupled, 50 mW maximum)

Objectives

  • 16x 0.8 NA Nikon

  • 20x 1.0 NA Zeiss

  • 40x 1.0 NA Zeiss

Cameras

  • 2x Hammamatsu Orca Flash 4.0

Limitations

  • Due to length of imaging experiments, fluorophores with excellent brightness and photon budget are required in order to maintain reasonable signal throughout imaging.

  • Cannot perform simultaneous multi-color imaging. Multiple color channels are acquired sequentially with a delay of 50 ms required for switching emission filters.

  • Spatial system resolution is less than that of the lattice light sheet microscope.

  • This is a diffraction limited instrument; it cannot perform super resolution imaging.