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Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) at the Advanced Imaging Center
Introduction Starting in August 2019, the Advanced Imaging Center (AIC) introduced its first pre-commercial electron microscope. This...
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7 min read
Obtaining data from the AIC is not the same as using the data from the AIC
What you should know about handling big data Written by Teng-Leong Chew and Eric Wait The era of “big data” in imaging With the recent...
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3 min read
How poor photon budget can ruin your experiments and your AIC proposal
Written by Teng-Leong Chew Photophysics of photobleaching A molecule will emit fluorescent light when its electron, falling from the...
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3 min read
Probes for single molecule localization microscopy
Written by Jesse Aaron Background The last 10 years has seen an explosion of techniques aimed at creating biological fluorescence images...
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6 min read
Understanding Objective Orientation in the LLSM
Written by John Heddleston How is orientation of the Lattice Light Sheet Microscope objectives different? As mentioned in an earlier...
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2 min read
What makes a successful proposal to the AIC?
Written by Teng-Leong Chew One of the most common questions we receive at the AIC is: what exactly constitutes a strong proposal – what...
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7 min read
Lattice Light Sheet Sample Prep
Written by John Heddleston The lattice light sheet microscope (LLSM) is a powerful optical tool for observing rapidly-occurring processes...
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5 min read
Thoughts on fluorescence recovery analysis
Written by Teng-Leong Chew The inherent tendency of fluorescent molecules to photobleach has been turned into a very powerful tool for...
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4 min read
Comparing Super-Resolution Techniques
Written by Jesse Aaron For many researchers, the prospects afforded by super-resolution (SR) imaging are both vast and exciting. The...
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8 min read
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